Compensation Quiz (part 1)

(Go back to the Introduction).

For a quick test of whether or not you know how to properly compensate a sample, answer three questions based on the figures shown.

In this hypothetical experiment, cells were stained with FITC CD3 and with a PE isotype control, and collected at different compensation values to correct for the FITC spillover into the PE channel (panel 1 is uncompensated; 2-5 are for increasing compensation values).

Figure 1A:

Quiz, Question #1: Which panel represents proper compensation?
Quiz, Question #2: On what basis did you make this determination?

After you have answered these questions, go to part 2 of the quiz.