The detailed discussion of compensation.

Introduction

Why do we need Compensation?

How does compensation work? (1-way)

How does compensation work? (2-way)

A mathematical example of 2-way compensation

Three-color compensation

Generalized compensation (n-colors)

Autofluorescence

Requirements for proper compensation

Co-stained compensation samples

2-color: examples (& what to look for)

An example of bad 3-color compensation

Errors introduced by compensation

Postscript

References

 

Go to the informal discussion.