The excitation spectrum and emission spectra (bold line) are shown for
Fluorescein (FITC). The red-shaded region represents the bandpass filter
commonly used to measure FITC fluorescence. On most flow cytometers, FITC
is excited by an argon laser (tuned to 488 nm)
.
Compare the FITC fluorescence spectra to spectra
for several FACS immunofluorescence dyes.