Allophycocyanin (APC)

The excitation spectrum and emission spectra (bold line) are shown for APC. The red-shaded region represents the bandpass filter commonly used to measure APC fluorescence. On most flow cytometers, APC is excited either by a dye laser tuned to 595-605 nm or by a HeNe laser tuned to 633 nm.

Also see the absorbance spectrum for APC.

Compare the APC fluorescence spectra to spectra for several FACS immunofluorescence dyes.